Data supporting "Detecting bumble bees in the wild using environmental DNA: development and validation of a qPCR assay for the endangered Franklin's bumble bee (Bombus franklini)"

This dataset includes quantitative polymerase chain reaction (qPCR) data for an in vitro test of the Bombus franklini eDNA assay using target and nontarget DNA extract, qPCR data for a plate used to calculate the limits of detection and quantification of the assay based on serial 10-fold dilutions of B. franklini gBlock, qPCR data for an in situ test of the assay using flower samples collected within and around the historic range of B. franklini (including extraction negatives, qPCR no-template controls, and standards), and metabarcoding data for the same flower samples (including extraction negatives, mock samples, and metabarcoding no-template controls).