Green Synthesis of Silver Nanoparticles Using Murraya Koenigii: Characterization, Antioxidant, and Antibiofilm Potential

This study investigates the green synthesis of silver nanoparticles (Ag-NPs) utilizing the leaf extract of Murraya koenigii as reducing and capping agent. The synthesized nanoparticles were characterized using various analytical techniques, including UV–Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and X-ray Diffraction (XRD). The successful formation of AgNPs was confirmed by the appearance of a distinct surface plasmon resonance peak at 420 nm. Morphological analyses revealed that the nanoparticles predominantly spherical shape, with particle sizes range from 25 and 50 nm. Phytochemicals present in leaf extract played an important role in reducing silver ions and stabilizing nanoparticles. The biological activities of synthesized Ag-NPs were evaluated viz antioxidant and antibiofilm assays. Antioxidant potentials determined using hydrogen peroxide scavenging assay, while antibiofilm activity was assessed using tissue culture plate method against Staphylococcus aureus (MTCC 1678) and Escherichia coli (MTCC 737). The findings indicated that biosynthesized AgNPs exhibited considerable antioxidant and antibiofilm activities, suggesting their potential application as effective anti-microbial and bio medical agents.