Screening of a Cyanobacterium Lyngbya bipunctata Lemm. for Antibacterial Potential

Cyanobacterium, Lyngbya bipunctata, was isolated from the soil samples collected from the Maharashtra state from the locations Ahilyanagar, India, in August 2024. For the identification, morphological variation, and taxonomical methods consistent. The axenic culture of Lyngbya bipunctata was obtained by way of the use of the techniques. The isolated Lyngbya bipunctata was autotropically cultured in BG-11 medium and incubated at 30±2°c. After 25 days, biomass was harvested by means of filtration through double-layered muslin cloth and dried with the help of air blower. The biomass of this Lyngbya bipunctata species was used for the evaluation of antibacterial potential against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus, and Proteus mirabilis. The antibacterial property was evaluated by the disc diffusion method. Methanol extract of Lyngbya bipunctata confirmed the antibacterial property against all the tested bacterial strains. Maximum zone of inhibition (22±1.4 mm) was observed in the methanol extract of Lyngbya bipunctata against Bacillus subtilis. The MIC for all the tested strains of bacteria was found in between 32 and 256 µg/ml.