CHANGES IN THE HISTOARCHITECTURAL STRUCTURE OF THE RAT SPLEEN ONE HOUR AFTER ADMINISTRATION OF LEIURUS MACROCTENUS SCORPION VENOM

Structural changes in the spleen of rats were studied and analysed one hour after administration of Leiurus macroctenus scorpion venom. The aim of the study.To study structural changes in the spleen of rats 1 hour after administration of Leiurus macroctenus scorpion venom. Materials and methods.Experimental studies were conducted on 10 male rats (180 g ± 3 g) that were injected intramuscularly with 0.5 ml of the poison solution (28.8 mg/ml) (LD 50 = 0.08 mg/kg).For microscopic examination, spleen samples were taken from animals.Histological staining of spleen preparations was performed using hematoxylin-eosin and Azan trichrome. Results of the study.Histological studies of the spleen structure in rats following the introduction of Leiurus macroctenus venom, 1 hour after the start of the experiment, revealed early signs of organ damage.The spleen capsule is unevenly thickened with defibering of collagen bundles, deformation and disorganisation of the fibrous component of the trabeculae and spasm of the wall of the trabecular arteries.There is a violation of the clear demarcation of the white and red pulp; expansion of the lumen of the central arteries and trabecular veins; erythrocyte stasis and sludge syndrome phenomena are observed in the lumen of the vessels.In the centres of reproduction, a decrease in the number of lymphoblasts compared to the norm is noted.Karyorrhexis and the appearance of cellular detritus are noted, which is chaotically located among viable lymphocytes; expansion of the marginal zone, an increase in the number of erythrocytes migrating from the red pulp, and the number of medium lymphocytes and macrophages; phenomena of leukopedia due to the active exit of leukocytes from the vascular bed into the parenchyma.The splenic cords of Billroth are filled with a large number of erythrocytes, which indicates extravasation.An increased number of segmented neutrophils is observed, a classic response to acute stress and intoxication. Conclusions.According to the results of the histological study of the spleen of rats 1 hour after the administration of the venom of the scorpion Leiurus macroctenus, it was proven that under the influence of the venom, there is an expansion of the lumen of the central arteries and trabecular veins with erythrocyte stasis and sludge syndrome Журнал «Перспективи та інновації науки» № 2(60) 2026