Study the effect of low-power helium-neon laser (632.8nm) on the human red and white blood cells

The purpose of this experiment was to investigate the impact of laser radiation on certain blood characteristics of students. Blood samples were collected from healthy individuals who were normal students, under standard laboratory conditions. A total of twenty healthy were obtained. Each sample was divided into three parts, with one part serving as a control (untreated) and the other two parts being exposed to laser radiation with power levels of 0.3mW and 0.9mW. The blood samples were irradiated using a continuous helium-neon laser with a wavelength of 632.8 nm for a duration of 40 minutes. Blood analysis was conducted using a complete blood count (CBC) device, and the results of the exposed samples were compared to the control samples. After 40 minutes, the CBC analysis showed changes in the components and characteristics of the blood. The number of white blood cells significantly increased with higher power levels, and there was also an increase in the number of platelets. Random fluctuations in the sedimentation rate of hemoglobin and red blood cells were observed. However, statistical analysis of the average counts of white blood cells, red blood cells, and platelets for all groups indicated that the observed increases were not statistically significant (p > 0.05). This emphasizes the importance of low-power lasers in various biomedical applications for treating different illnesses. The study concluded that laser radiation altered the biological components of the irradiated samples.