CHEMICAL COMPOSITION OF HYDROLYZATE OF AQUEOUS EXTRACT OF SALVIA GAREDJI

The aqueous extract of the aerial parts of the Caucasian endemic plant Salvia garedji Troitzk. (family - Lamiaceae Lindl.) is characterized by an abundant content of phenolic compounds (49.8% tannin by permanganometry). To determine the nature of polyphenols, acidic hydrolysis of the aqueous extract was performed. HPLC-MS analysis of the hydrolysate revealed the presence of phenolic acids, their derivatives, terpene phenols, and flavonoids (a total of 32 substances). 13 of them - carnosol, carnosic acid, yunnaneic acid F, sagerinic acid, salvianolic acid A, salvianolic acid A isomer, luteolin-7-0-glucuronide, apigenin-7-0-glucuronide, rosmarinic acid, methyl gallate, cirsimaritin, epigallocatechin, danshensu (salvianic acid A) - were previously identified in the native aqueous extract. 19 substances - rosmanol, salvianolic acid F, rosmaridiphenol, caffeoyl threonic acid, p-hydroxybenzoyl glucose, caftaric acid, miltirone, ethyl rosmarinate, yunnaneic acid E, yunnaneic acid D, eriodictyol-di-glucoside, rosmarinic acid isomer, yunnaneic acid H, cirsilineol, feruloyl tartrate (fertaric acid), 6-hydroxy-luteolin-7-O-glucuronide, chrysin-7-glucuronide, 5-hydroxy-6,7,3′,4′-tetramethoxyflavone, 3-O-feruloylquinic acid - are products of hydrolysis. The absence of gallic and ellagic acid residues in the hydrolysate indicates that the extract does not contain hydrolysable tannins. Therefore, the result (49.8 %) obtained by the permanganometric titration method for the quantitative determination of tannin substances in plant raw materials given in the State Pharmacopoeia is actually due to the oxidation of polyphenols of another groups. In various in vitro experiments, the aqueous extract showed high antioxidant activity.