Combination of Temu Kunci Extract with Sirsak Leaf Extract Using Ultrasonic Maceration as a Candidate for Cervical Cancer (HeLa)

Cervical cancer remains a leading cause of cancer-related mortality among women, primarily linked to Human Papillomavirus (HPV) types 16 and 18. Conventional treatments such as chemotherapy and radiotherapy are often limited by adverse effects and high costs, prompting the need for safer, natural-based alternatives. This study aimed to evaluate the anticancer potential of the combined ethanolic extracts of fingerroot (Boesenbergia pandurata Roxb.) and soursop leaves (Annona muricata L.) obtained through ultrasonic maceration as a candidate therapy for cervical cancer (HeLa). The study included plant determination, simplicia preparation, ultrasonic extraction using 96% ethanol, phytochemical screening, quantification of total flavonoid and phenolic contents, and cytotoxicity testing via MTT assay on HeLa cells. Data were analyzed by calculating IC₅₀ and Combination Index (CI) values using SigmaPlot software. The results revealed that fingerroot and soursop extracts exhibited cytotoxic activity with IC₅₀ values of 59.71 µg/mL fingerroot and 94.55 µg/mL soursop, respectively, while their combination showed synergistic effects (CI < 1). The conclusion of this study is that the ultrasonic maceration method shows that ginger and soursop leaf extracts have cytotoxic activity against cervical cancer cells (HeLa). The combination of the two extracts shows a strong synergistic effect against cervical cancer cells (HeLa) with a CI value of 0.365, making it a promising candidate for anticancer phytotherapy. Keywords: Cervical Cancer, Boesenbergia Pandurata, Annona Muricata, Ultrasonic Maceration, Synergistic .