Evaluation of subchronic toxicity of a phytosorption preparation based on dihydroquercetin

The article discusses a preclinical investigation into the subchronic toxicity of a novel phytosorbent drug developed by the Pharmacology and Toxicology Department at Saint Petersburg State University of Veterinary Medicine. This drug, which contains dihydroquercetin, is intended for use in the prevention and management of metabolic disorders of the liver and biliary system in fish. The study demonstrated that the drug possesses sorption and hepatoprotective characteristics, which are significant in maintaining metabolic equilibrium in fish under contemporary conditions of increased human activity and enhanced aquaculture. The tests were conducted in accordance with internationally recognized standards for preclinical investigations and the regulations of the Russian Federation. Wistar-strain laboratory rats were utilized, which were maintained under strict monitoring conditions. The rats were administered the drug for a period of 21 days, at a dosage of 2000 mg/kg body weight. Assessment of subchronic toxicity was based on pre- and post-experiment blood test outcomes. Statistical analysis was conducted on the acquired data. Clinical and biochemical analyses failed to detect any toxic impacts, as the indices of the experimental group resembled those of the control group. Some abnormalities were observed, including decreases in red blood cell count, hemoglobin levels, and creatinine levels, as well as increases in platelet count and alkaline phosphatase levels. These changes do not suggest toxicity and may be related to the adsorption properties of the medication or irritation of the gastrointestinal lining. The decrease in bilirubin and ALT levels indicates a pharmacological response associated with hepatoprotection. Although the medication was found to be generally safe, further research into dose-dependent effects is warranted.