Peer Review Report For: Amino Acid and Fatty Acid Profiles of Black Soldier Fly Larvae Meal Reared on Rice Bran, Milk Waste, and Slaughterhouse Waste Combined Substrate Under Different Processing Methods [version 1; peer review: 1 approved with reservations]

Background Black Soldier Fly (BSF) larvae is a promising alternative source for animal feed due to their ability to convert organic waste into high-quality biomass rich in essential amino acids and beneficial fatty acids. However, the post-harvest processing method is crucial affecting nutritional value. This study evaluated the effects of five processing methods (air-drying, sun-drying, oven-drying, microwave-drying, and roasting) on the amino acid and fatty acid profiles of BSF larvae reared on rice bran, milk waste, and slaughterhouse waste combined substrate. Methods BSF larvae were reared for seven days on a combined substrate consisting of rice bran, milk waste, and slaughterhouse waste. After harvesting, the larvae underwent five different drying treatments until a constant weight was achieved. The dried larvae were ground into powder and analyzed for amino acid and fatty acid profiles. Results BSF larvae meal contained substantial amounts of essential amino acids, particularly lysine (1.76–2.41%), leucine (1.46–2.11%), and valine (1.41–1.77%). Fatty acid profiles of BSF larvae meal were predominantly composed of lauric acid (22.07–24.88%), palmitoleic acid (22.84–27.89%), and linolelaidic acid (20.95–23.86%). Microwave drying retained the highest levels of essential amino acids and fatty acids. Oven drying provided balanced retention, while roasting effectively preserved key fatty acids but posed a risk of amino acid degradation. Sun drying and air-drying maintained monounsaturated fatty acids and key amino acids, offering a cost-effective alternative despite the prolonged drying period. Conclusions Microwave drying is the most effective method for preserving the nutritional quality of BSF larvae meal, but other methods offer practical advantages depending on resource availability and processing goals. Future research should explore hybrid drying techniques, optimization of processing conditions, and long-term stability assessments to enhance the efficiency and applicability of BSF larvae meal as a sustainable feed ingredient.