Peer Review Report For: The Genetic Association of Single Nucleotide Polymorphisms of SERPINE1 (rs6092) and IFNAR2 (rs1051393, rs2229207) Genes Is Related to Post Covid-19 Respiratory Syndrome [version 1; peer review: 1 approved with reservations]

Background Coronavirus disease 2019 (Covid-19) is a disease of the respiratory system caused by the SARS-CoV-2 virus. The severity of Covid-19 can be affected by the presence of single nucleotide polymorphisms (SNPs) in certain genes, such as IFNAR2 and SERPINE-1. This study aimed to identify and analyze the presence of SNP rs6092 in the SERPINE1 gene and rs1051393 and rs2229207 in the IFNAR2 gene relation in Covid-19 respiratory syndrome in Indonesia. Methods DNA was isolated from saliva samples of all patients, and a TaqMan Genotyping Assay with a real-time PCR instrument was used to run the samples. The output data were analyzed for demographic data, allele frequency, genotype frequency, and the association of all SNPs with the Covid-19 respiratory syndrome (case) and control subjects. We also analyzed blood laboratory results, blood gas analyses, coagulation factors, and inflammatory factors using SPSS. Results This study included 85 subjects comprise with Covid-19 respiratory syndrome and control subjects. Our study found no association between subjects with Covid-19 respiratory syndrome and any of the variants. However, based on the symptoms caused by rs1051393, we found that it had an effect on fever symptoms. In addition, a significant relationship between rs2229207 and chest pain symptoms was observed in patients with case group. Furthermore, our study found significant differences (p < 0.05) in several blood laboratory analyses, such as the level of basophils and eGFR for rs6092 and the potassium level for rs2229207. Furthermore, arterial blood gas analysis showed that pCO2 and pH levels were significantly different for rs2229207. Conclusion Our study found an association between rs1051393 and fever and between rs2229207 and chest pain in patients with post Covid-19 respiratory syndrome.