Analysis of Physiochemical Parameters and Identification of Fatty Acids by Gas Chromatography: Mass Spectroscopy (GC-MS) Analysis in Comparison with Cow Ghee and Shata-Dhauta-Ghrita

Skin diseases remain a prevalent issue despite significant advances in diagnosis and treatment. Ayurveda, with its deep understanding of skin disorders, offers promising solutions, particularly through formulations like Shata- Dhauta-Ghrita (SDG). This Ayurvedic remedy is produced by washing pure cow ghee repeatedly 100 times in a copper vessel, resulting in a product with enhanced cell regeneration and healing properties. SDG is effective for treating various skin conditions such as burns, scars, cuts, shingles, and herpes. The process of washing ghee refines it into a more absorbable form with improved moisturizing and scar-healing abilities. SDG’s improved permeation properties make it a superior option for topical applications compared to pure cow ghee, allowing for better absorption, deeper action, and enhanced therapeutic benefits. The purpose of this study was to study the physiochemical evaluation as well as identification of fatty acids is carried out using Gas Chromatography-Mass Spectrometry (GC-MS) analysis in comparison to cow ghee and Shata-Dhauta-Ghrita. The pure cow ghee used for the preparation of Shata-Dhauta-Ghrita was sourced from ‘Shri Shri Radha Goverdhandhari Go Shala’, Nashik, Maharashtra, India. The SDG is evaluated for organoleptic tests, which included assessments of color, odor, taste, appearance, and texture. Additionally, physicochemical properties such as pH, saponification number, iodine number, peroxide value, viscosity, moisture content, copper content, unsaponifiable value and particle size were analyzed. The formulated SDG were also subjected Gas Chromatography-Mass Spectrometry (GC-MS) analysis to further evaluation of their chemical composition and potential efficacy. Shata-Dhauta-Ghrita found as a more refined and effective option for skin treatment, overcoming the limitations of pure cow ghee while retaining its natural benefits.