Differential Effects of Organ-Specific Xenogeneic Progenitor/Precursor Stem Cell Secretomes with Regeneration on Human Umbilical Vein Endothelial Cell Wound Healing

The regenerative potential of stem cell-derived secretomes has attracted increasing interest as a cell-free therapeutic strategy for tissue repair and vascular regeneration.Xenogeneic progenitor stem cells secrete a complex mixture of cytokines, growth factors, extracellular vesicles, and signaling molecules capable of modulating endothelial migration and wound healing responses.This study investigates the effects of conditioned supernatants derived from tissuespecific xenogeneic progenitor stem cell cultures on endothelial wound closure kinetics using a Human Umbilical Vein Endothelial Cell (HUVEC) scratch assay model.Conditioned supernatants obtained from Retina, Kidney, Brain, Heart, and Thymus progenitor/precursor stem cell cultures were applied to confluent HUVEC monolayers following standardized scratch induction.A negative control group receiving basal culture medium without stem cell supernatant supplementation was included for comparative analysis.Wound closure progression was monitored over defined incubation intervals using quantitative image-based analysis to assess endothelial migration and repair dynamics.