AlphaGenome/VirDLA-Guided Evolution of Compact Senescence-Responsive Promoters: Sequences and Supporting Data

This release includes compact senescence-responsive promoters that can be placed directly upstream of an open reading frame (ORF). Their senescence-responsive activity trends have been validated in dual-luciferase reporter assays.This update extends the earlier p16INK4a compact-promoter designs with compact synthetic promoters generated through AlphaGenome-guided genetic algorithms. The files accompany “Directed evolution of compact synthetic promoters via AlphaGenome and genetic algorithms” and include candidate and control sequences, genetic algorithm parameters, and VirDLA reporter cassette designs. Supplementary datasets provide experimental MPRA measurements paired with virtual assay predictions and predicted tissue activity profiles for the evolved promoters.