zazadovv/Macrophage_Vessel: Macrophage_Vessel

Blood vessels near injury sites rapidly dilate, become permeable, and releaseserum and leukocytes into the wounded tissue to support healing andregeneration. How the vasculature senses distant homeostatic tissue perturbationswithin seconds-to-minutes remains incompletely understood. Usinghigh-speed imaging of live zebrafish larvae, we monitor two hallmark vascularresponses to injury: vessel dilation and serum exudation. By genetic, pharmacologic,and osmotic perturbation along with leukocyte depletion, weshow that the cPla2 nuclear membrane mechanotransduction pathway convertsa ~ 50 μm/s osmotic wound signal into rapid vessel-permeabilizationvia perivascular macrophages, 5-lipoxygenase (Alox5a), and leukotrieneA4 hydrolase (Lta4h). By revealing a previously undescribed physiologicalfunction of nuclear membrane mechanotransduction, we provide real-timeinsights into the long-range communication of wounds and blood vessels inintact tissue.