Toxicity data for: COLLEMBOT: AI-based counting of Collembola for OECD 232 Tests

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Authors: Micha Wehrli, Adrian Meyer, Éverton Souza da Silva, Sam van Loon, Bart G van Hall, Cornelis A.M. van Gestel, Tiago Natal‐da‐Luz, Max V. R. Döring, Heike Feldhaar, Magdalena M. Mair, Denis Jordan, Miriam Langer
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Abstract

This dataset is part of a scientific article, COLLEMBOT: AI-based counting of Collembola for OECD 232 Tests, in Environmental Toxicology & Chemistry: https://doi.org/10.1093/etojnl/vgag068. This dataset contains raw and processed data from laboratory toxicity tests on Folsomia candida (Collembola) exposed to various soil contaminants under controlled conditions. The study compares traditional manual counting of juveniles and adults with automated image-based counting using the COLLEMBOT system. Data were collected across multiple soils (LUFA 2.2, OECD variants) and exposure scenarios, including different concentrations of pesticides (e.g., imidacloprid, chlorpyrifos, lindane), fungicides (fluazinam, cyproconazole), microplastics (polystyrene), and reference substances (boric acid). The dataset includes: Raw observations: survival and reproduction endpoints per replicate. Manual vs automated counts: paired measurements for validation of automated image analysis. Metadata: compound identity, soil type, concentration (nominal and adjusted), replicate information. Dose-response modeling script: ECx values (ED10–ED90), NOEC/LOEC estimates, and statistical comparisons between counting methods. This resource supports ecotoxicological research, automation in soil toxicity testing, and reproducibility in environmental risk assessment workflows. There is an online version available at Huggingface, we are happy to recieve feedback on the usability: https://huggingface.co/spaces/MichaWehrli/COLLEMBOT_BETA_v0.1.0_under_development This repository also contains the model weights for COLLEMBOT under a AGPL 3.0 license with the code being available at GitHub under a MIT license: https://github.com/waldstrom/collembot

Fields of Study & Subjects

Environmental Toxicology and Ecotoxicology Collembola Taxonomy and Ecology Studies Smart Agriculture and AI

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
16 January 2026
Document type
Dataset