Ratio Imaging Analyzer (RIA): A Standalone, Zero-Dependency Tool for Robust Ratiometric Fluorescence Analysis (v2.0.1)

Ratio Imaging Analyzer (RIA) v2.0.0 is an open-source desktop application designed to streamline the processing of ratiometric fluorescence imaging data (e.g., FRET, Calcium, pH sensors). v2.0.0 Update Highlights:To balance portability with professional power, RIA is now available in two editions: ⚡ RIA Lite (Standalone EXE): A re-engineered, ultra-compact executable optimized for instant startup and maximum portability on standard Windows PCs. 💎 RIA Pro (Python/PyPI): The full-featured Python version that integrates comprehensive dependencies including Olympus .oir, Nikon .nd2, and Zeiss .czi. Key Features: 🧠 Robust Motion Correction: Integrates the Enhanced Correlation Coefficient (ECC) algorithm. 📉 Scientific-Grade Signal Processing: Implements custom Normalized Convolution to handle NaN values correctly. ✨ High-Contrast ROI System: Features a custom multi-layered rendering engine. 📊 Analysis Unchained: Allows rigorous background subtraction and time-series extraction on personal laptops.