First Reported Application of Calcium Ionophore at the Human Cleavage Stage: A Case Report

Abstract Background: Calcium ionophore (A23187) is commonly used in assisted reproductive technology, where it has shown significant potential to enhance fertilization rates and embryo development. It facilitates the movement of calcium ions across cell membranes, thereby manipulating the intracellular calcium signaling in sperm and oocytes. The efficiency and safety of calcium ionophore (A23187) activation, its effects on embryo developmental potential, and its effects on pregnancy outcomes after embryo transfer have been approved. Case: A 32-year-old woman was first seen at our hospital in January 2025. She presented with a history of secondary infertility for the past 8 months and has no living children. She experienced three spontaneous pregnancies and one pregnancy following ovarian stimulation, but all resulted in chemical pregnancies. Her hormonal profile indicated a polycystic ovary case with normal FSH, LH, and Prolactin. Conclusion: Our findings demonstrate that Calcium Ionophore (A23187) activation is a safe and effective strategy for enhancing blastocyst formation and euploidy embryos. Keywords: Calcium Ionophore (A23187); ICSI; AOA; cleavage stage embryos; blastocysts); Euploidy.