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The heavy metal pollution is increasing many folds due to human activities especially for wide use of manganese. Thus, it is important to manage its pollution by biological mean. In the present study Tadoba forest soil of regions Agarzari road and Junona kuti sampled for manganese tolerant bacteria serially diluted for making it as inoculum source. To screen manganese tolerant bacterial species 1/10 concentration of LB media supplemented with 0.05, 0.1, 0.5, and 1 mM MnSO4.H2O as stressor heavy metal and upon inoculation plates were incubated at 37°C for 72-96 hrs. Developed colonies considered as tolerant species and two of them screened for IMViC, sugar fermentation and identified by 16s rRNA gene sequencing. Further the culture was analyzed for its MIC by screening them with MnSO4.H2O dose in the range of 0.1 µg to 100 µg/ml. Further the manganese tolerant isolates screened for growth curve to ascertain the possible change in log phase, stationary phase and death phase transition once compared to control for manganese stressed condition. In the result section, we recorded the soils of Tadoba forest remained prominent in manganese tolerating bacteria and many isolates abled to tolerate the maximum dose as 1 mg/ml and among those two isolates identified as Klebsiella pneumoniae 1/AR/MnSO4 and other as B. licheniformis 1/JK/MnSO4 as per 16S rRNA sequencing. Both isolates found to be Indole (+ve), methyl red (+ve), VP (-ve), and citrate (+ve) and abled to ferment all sugars tested as glucose, adonitol, arabinose, lactose, sorbitol, mannitol, rhamnose, and sucrose.