Microbial selenoproteins in ulcerative colitis using long-read metagenomics and deep learning

The Oxford Nanopore sequencing data generated and analysed in this study along with all metadata and supplementary tables are available in Zenodo at 10.5281/zenodo.21390625. The following folders/files are present in the repository · 16S_rRNA_raw_fastq.rar: 16S rRNA gene amplicon data (basecalled fastq) from 9 faecal samples · Metadata_MES0_MES3.xlsx: Containing all amplicon related metadata. · Metagenomes_raw.zip: Assembled metagenomes from the nine representative patients. · Deep-Sep_output.zip: Table S1: deep-Sep output containing selenoprotein hits, MAG reference, protein sequence and taxonomy for MES0; Table S2: deep-Sep output containing selenoprotein hits, MAG reference, protein sequence and taxonomy for MES3; Table S3: deep-Sep output containing selenoprotein hits, MAG reference, protein sequence and taxonomy for HC. · COG_classify_results.zip: Table S4: List of COGs predicted from input selenoprotein hits from MES0; Table S5: List of COGs predicted from input selenoprotein hits from MES3; Table S6: List of COGs predicted from input selenoprotein hits from HC. · MFE_validation.zip: Table S7: MFE analysis comparison between randomly shuffled sequences with same GC identity. Table S8: MFE analysis comparison between genomic sequences with same GC identity. · MFE_validation.zip: Table S9: RNA-folding structure validation.