CellSens vsi files (original and deconvolved) for testing of floating point data conversion

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Authors: Barbora Hyklova, Iva Švecová
Cited by: 0

Abstract

The dataset contains CellSens .vsi files of pollen seeds from Olympus SpinSR10 microscoscope obtained in super-resolution mode (additional 3.2x magnification) with the 40x/0.95 objective. The images with 20MLE in name were deconvolved using CellSens 4.4.1 Constrained Iterative method for Olympus Super Resolution on spinning disk. After deconvolution, the intensities are displayed as floating point values in CellSens, but the same data loaded with Bio-Formats to Fiji or Python (bioio-bioformats) are integer and scaled compared to CellSens, with the scaling factor different for each image. The problem is discussed on image.sc with more details: https://forum.image.sc/t/cellsens-deconvolved-images-vsi-float-loaded-with-inconsistent-intensity-scaling-via-bio-formats

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
6 January 2026