Image datasets from 'Optimised Protocols for Live imaging of Bacillus subtilis phage infection at a single-cell resolution'
Abstract
List of uploaded files, that were described in 'Optimised Protocols for Live imaging of Bacillus subtilis phage infection at a single-cell resolution'; All files are linked to 'Additional protocols - Image postprocessing': Original time series: time_series_raw_data_positions_1_6.czi - raw czi file – original recorded time-series, containing 6 positions in the sample. Unprocessed. Further processing performed on position #1 (see files below). Control images: bacteria_mkate_green.tif - control image [see Bacteria_only_G] bacteria_mkate_green_scale.jpg - control image with scale bar added bacteria_mkate_red.tif - control image [see Bacteria_only_R] bacteria_mkate_red_scale.jpg - control image with scale bar added phage_SYBR_Gold_green.tif - control image [see Phage_only_G] phage_SYBR_Gold_green_scale.jpg - control image with scale bar added Partly processed time-series (position #1) before and after removal of background fluorescence. Splitted channels: G_250801_p1_bacteria_phage.tif - green channel before background removal G_BS_250801_p1_bacteria_phage.tif - green channel after background removal [see G_BS_ date_p#_sample] R_250801_p1_bacteria_phage.tif - red channel before background removal R_BS_250801_p1_bacteria_phage.tif - red channel after background removal [see R_BS_ date_p#_sample] Partly processed time-series (position #1) - generated scaled time-series for cross-talk background removal: R_scale_α.tif - R_BS_250801_p1_bacteria_phage images after multiplying by α value (0.1358) R_scale_β.tif - R_BS_250801_p1_bacteria_phage images after multiplying by β value (0.0067) Partly processed time-series (position #1) - green channel after background and cross-talk removal: G_BS_CTR_250801_p1_bacteria_phage.tif - green channel after background and cross-talk removal [see G_BS_CTR_ date_p#_sample] Fully processed time-series (position #1) - merged channels after background removal: processed_time_series_merged_position_1.tif - R_BS_250801_p1_bacteria_phage.tif and G_BS_CTR_250801_p1_bacteria_phage.tif merged
Fields of Study & Subjects
Bacteriophages and microbial interactions
Cell Image Analysis Techniques
Advanced Fluorescence Microscopy Techniques
Publication details
- Journal / source
- Zenodo (CERN European Organization for Nuclear Research)
- Publication date
- 7 January 2026