Phylogenetic analysis and non-structural protein 2 (Nsp2) amino acid sequence alignment of the porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) strain Lleida 029_22.

(A) Maximum likelihood phylogenetic tree constructed from whole-genome sequences using the general time reversible model and 1000 bootstrap replications. The Lleida 029_22 strain used in this study is indicated by a red triangle and representative strains collected during a Rosalia-related outbreak are indicated with a circle. (B) Partial Nsp2 amino acid sequence alignment of Lleida 029_22, representative strains collected during a Rosalia-related outbreak, the high-virulence PR40 strain, and the prototype strain, Lelystad. Sequence alignment was performed using CLC Genomics Workbench 24.0.1.