A 178bp diagnostic D-loop fragment (OvisCR) to discriminate domestic sheep (Ovis aries) and European mouflon (Ovis musimon) in diet metabarcoding studies

Supplementary Material for : A 178bp diagnostic D-loop fragment (OvisCR) to discriminate domestic sheep (Ovis aries) and European mouflon (Ovis musimon) in diet metabarcoding studies. Table of contents Supplementary Material 1. DNA quantification protocols. Supplementary Material 2. Functions and parameters used in the metabarcoding pipelines. Supplementary Material 3. Diagnostic Dloop fragment (OvisCR) haplotypes (178pb) for 92 Ovis reference samples collected in France. Supplementary Material 4. TCS haplotype network based on a 674 bp D-loop fragment, constructed from 30 Ovis musimon and 41 Ovis aries reference samples collected in France. Supplementary Material 5. Metabarcoding results of 115 wolf scat samples collected in France and sequenced using the 12S and OvisCR fragments, analysed with two bioinformatic pipelines (OBITools and DADA2). Supplementary Material 6. OvisCR reference database Supplementary Material 7. ASV table for the 12S fragment obtained from metabarcoding of 115 wolf scat samples collected in France and analysed with DADA2 Supplementary Material 8. ASV table for the OvisCR fragment obtained from metabarcoding of 115 wolf scat samples collected in France and analysed with DADA2 Supplementary Material 9. OTU table for the 12S fragment obtained from metabarcoding of 115 wolf scat samples collected in France and analysed with OBITools Supplementary Material 10. OTU table for the OvisCR fragment obtained from metabarcoding of 115 wolf scat samples collected in France and analysed with OBITools