Assessment of recombinant soluble Glycosylated Protein 3 (sGP3) -binding antibody depletion on porcine reproductive and respiratory syndrome virus-1 (PRRSV-1) neutralization

Serum from hyperimmune pig 64 (A, C) and naïve pig serum (B, D) were each depleted under three conditions: using recombinant PRRSV-1 sGP3; recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein receptor-binding domain (S-RBD) (irrelevant control); or mock depletion without antigen. The effect of antibody depletion was assessed by sGP3 ELISA (A, B) and virus neutralization titer (VNT) (C, D). Mean data from two technical replicates ± SD are shown