Assessment of concentration and specificity of porcine monoclonal antibodies (mAbs) in culture supernatants from transfected Expi293F cells

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Authors: Jack W. P. Hayes, Simon Paul Graham
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Abstract

Following transfection, supernatants were screened by ELISA using an anti-pig Immunoglobulin G (IgG) (H+L) secondary antibody, and Optical Density (OD) values interpolated against a porcine IgG standard curve to determine concentration (A). Supernatants were also evaluated by ELISA for binding to PRRSV-1 sGP3 (B).

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
8 January 2026