DataSet for "Path integration guides homing in a coral reef fish"

We provide all raw data and code used in "Path integration guides homing in a coral reef fish" . Please read the README.txt file for a full description of the codes and datasets, including column information. See below for the methods used to collect the raw data and the statistical analyses description. Methods Overview We tested whether domino damsel fish can navigate back to a shelter using path integration and whether idiothetic path integration or landmark cues were favoured when the two cues gave conflicting information. We first allowed individuals to acclimatise overnight to a large circular experimental apparatus (i.e. pool) with two landmarks and a shelter. The next morning, we placed a trap with food rewards in the centre of the pool and a spotlight outside the curtain surrounding the pool. Fish were forced to leave their shelter in order to reach the food reward in the centre of the apparatus. Once trapped, we either (1) removed the landmarks and shelter from the pool; (2) removed the shelter and kept the landmarks; (3) removed the shelter and shifted the landmarks position by 90 degrees; (4 and 5) repeated tests 1 and 2 with an additional shift to the spotlight. Fish were then released from the trap, and free navigation movements were recorded and analysed. Ethics This study was reviewed and approved by the AWERB ethical approval (Ref. No. APA/1/5/ZOO/NASPA/Burt/Turbidity). Animal Husbandry We used 19 domino damselfish (Dascyllus trimaculatus), sourced from national suppliers (Tropical Marine Centre, Hertfordshire, WD3 5SX and The Goldfish Bowl, Oxford OX4 1RQ) in experiments. Individuals were wild-caught and kept by the supplier for a minimum of one month before being housed in the laboratory. We housed these territorial individuals in separate 0.35 m x 0. 32 m x 0.60 m tanks within a single flow-through marine system. We enriched each tank with 1 cm depth of coral gravel substrate, a large rock, a cave shelter (13 cm x 12 cm x 8 cm Aqua One Marble Cave), and a 30 cm tall plastic plant. The fish were kept on a 12-hour light/12-hour dark cycle with fluorescent light, and fed with crisp (TetraPRO Energy Multi-Crisps) in the morning and mysis shrimp in the afternoon to provide supplementary nutrients. We maintained water parameters at healthy levels for this species (Temperature: 26.0 °C, Salinity-specific gravity: 1.024, pH: 8.2, KH: 7-8 dKH, Nitrite: 0 ppm, Ammonia: 0 ppm, Nitrate: