A Review on HPTLC Method Development and Validation for Quantitative Estimation of Gliclazide

One second-generation sulfonylurea that is frequently given to treat type 2 diabetes mellitus is gliclazide. Reliable analytical techniques are crucial for guaranteeing the efficacy, safety, and purity of gliclazide in pharmaceutical formulations. For drug analysis, High-Performance Thin-Layer Chromatography (HPTLC) has become widely accepted as a quick, easy, accurate, and economical method. An overview of documented HPTLC techniques created and approved for the quantitative measurement of gliclazide, both alone and in conjunction with other antidiabetic medications, is provided in this paper. Along with validation parameters like accuracy, precision, linearity, specificity, robustness, and sensitivity in compliance with regulatory guidelines, important aspects of method development are covered, including the choice of stationary and mobile phases, detection wavelength, and chromatographic conditions. The review also emphasizes the useful benefits of HPTLC, including its minimal solvent consumption, capacity to analyze numerous samples at once, and suitability for regular quality control. All things considered, this review is a helpful resource for academics and analysts working on gliclazide analysis using HPTLC.