Evaluation Of Antidiarrheal Activity of a Prebiotic Formulation Containing (Gum Acacia, Gir Cow Ghee & Sugar) On Rat Model

The current work examines the in-vivo antidiarrheal effects of a prebiotic formulation containing gum acacia, Gir cow ghee, and sugar in a castor oil-induced diarrhea model with Sprague Dawley rats. Diarrhea was produced using castor oil (2 mL/kg), and the formulation's protective effects were assessed at two dose levels and compared to the conventional medicine loperamide (5 mg/kg). The following parameters were evaluated: beginning of diarrhea, total number of fecal outputs, wet feces, and percentage inhibition of diarrhea. The prebiotic formulation dramatically reduced diarrhea frequency and improved stool consistency in treated groups, with the high-dose group demonstrating the greatest inhibition. The data indicate that the synergistic impact of gum acacia (prebiotic fiber), Gir bovine ghee (a source of SCFAs, particularly butyrate), and glucose (which improves water-electrolyte absorption) helps to restore intestinal balance and prevent castor-oil-induced diarrhea. This study supports the use of natural, food-grade prebiotic compounds as a safe and effective antidiarrheal medication.