Xestochironomus luteifurcatus Sublette & Wirth 1972

Xestochironomus luteifurcatus Sublette & Wirth, 1972 Xestochironomus luteifurcatus Sublette & Wirth, 1972: 14, fig. 15. 1 Examined material. • 3 males (+ 2 voucher). Dominican Republic, Blanco, 18°52'42.7"N, 70°30'25.7"W, 30. xi. 2019, light trap, A. Höcherl, leg. (EBD). • 2 males. Dominican Republic, Blanco, 18°52'42.7"N, 70°30'25.7"W, 30. xi. 2019, light trap, A. Höcherl, leg. (MNHN-EJM). • 2 males, Blanco, 18°52'59.9"N, 70°30'28.8"W, 01. xii. 2019, Malaise trap. A. Höcherl, leg. (LEA). • 1 female, voucher, Blanco, 18°52'42.7"N, 70°30'25.7"W, 30. xi. 2019, light trap, A. Höcherl, leg. (EBD). Genbank sequences PV 761057 – PV 761058, PV 761061 – PV 761064. Redescription. Male (n = 9, except when stated otherwise). Total length 2.78–3.54, 3.07 mm. Wing length 1.48–1.79, 1.55 mm (8). Total length / wing length 1.97–2.15 (7). Wing length / profemur length 1.82–2.02 (6). Coloration. Completely light yellow greenish and unmarked (Fig. 4 A – F). Wing hyaline. Head (Fig. 4 A). AR 1.31–1.47 (7). Clypeus with 6–9 (3) setae. Tentorium 114–138 (6) μm long. Palpomere lengths (1–5 in μm; n = 5): 30–37, 34; 39–52, 45; 124–151, 138; 119–130, 125; 201–215, 210. Thorax (Fig. 4 B). Acrostichals 10–13, 12 (7). Dorsocentrals 13–29, 16 (8), partly biserial. Prealars 4 (8). Scutellum with 7–10, 9 (8) setae. Wing (Fig. 4 C, n = 3). Venarum ratio (VR) = 1.22–1.27. Brachiolum with 2–3 setae. R with 29–31 setae; R 1 with 20–28 setae; R 4 + 5 with 37–52 setae; RM with 2–3 setae; M with 4–8 setae apically. Squama with 5–8 setae. Legs (Fig. 4 E). Combs of midtibia 19–21 (4) μm and 21–22 (4) μm long spurs. Combs of hind tibia 18–19 (4) μm and 17–24 (4) μm long spurs. Width at apex of foretibia 43–4 6 (4) μm, of midtibia 42–52 (4) μm, of hind tibia 48–52 (4) μm. Lengths (in μm) and proportions of legs in Table 3. Hypopygium (Fig. 4 F). Tergite IX with 10–12 strong median setae and along the posterior margin several fine setae, on the ventral side. Anal tergite bands V-shaped, separated. Laterosternite with 2–3 setae. Anal point slender, 47–66, 53 (n = 5) μm long. Phallapodeme 50–76 (2) μm long. Transverse sternapodeme, 20–28 (3) μm long. Gonocoxite 75–113, 93 (7) μm long. Superior volsella hooked, 52–86, 75 (7) μm long. Inferior volsella 64–119, 102 (8) μm long. Gonostylus bifid, 117–141, 127 (7) μm long, apex of gonostylus markedly forked, with deep concavity between thumb-like lobes. Lateral lobe of gonostylus overreaching mesial lobe. HR 0.59–0.84 (7). HV 2.38–2.69 (7). Redescription of female (n = 1). Total length 2.43 mm. Wing bent, no observations possible. Coloration similar to male. Head. Lengths of flagellomeres (μm): 52, 55, 82, 87, 111. Temporals setae 14. Clypeus with 19 setae. Tentorium = 132 μm. Lengths of palpomeres (in μm). 29, 40, 223, 152, 236. Thorax. Acrostichals 10; dorsocentrals 18, partly biserial; prealar 4; scutellars 13. Wing. Squama with 7 setae. Legs. Combs of midtibia 15 μm and 29 μm long spurs. Combs of hind tibia 16 μm and 30 μm long spurs. Width at apex of foretibia 59 μm, of midtibia 37 μm, of hind tibia 49 μm. Lengths (in μm) and proportions of legs in Table 4. Genitalia. In a lateral position. Gonocoxite IX with 7 setae. Gonapophysis IX notum 273 μm long. Seminal capsules spherical, width 63 μm. Gonocoxapodeme with 150 μm. Coxosternapodeme with 103 μm. Cercus width 96 μm. Immatures. Unknown. Distribution. Dominica, but expanded to the Dominican Republic. Barcode of the Dominican Republic Xestochironomus We have conducted BLAST in GenBank. We have also conducted “ Identification ” query with tree-based search in BOLD v. 4. Search queries were conducted for the sequences of the five specimens of two species of Dominican Republic Xestochironomus species. No close matches (> 85 %) were found, with the closest coming from unidentified representatives of Chironomini from South and Central America. We think that the absence of the close matches is caused by the lack the Xestochironomus sequences in the databases (except one from Cranston et al. 2012, but that uses from different primers). In the neighbor-joining tree (Fig. 5), we found that the male and female of X. digitulus sp. nov. grouped with 99.8 % bootstrap support, and that the male and female of X. luteifurcatus grouped at 99.2 % bootstrap support. Genetic distances among the analysed species are shown in Table 5.