tkatsine/Counting-protein-aggregates: Counting protein aggregates

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Authors: tkatsine
Cited by: 0

Abstract

This is an open-source ImageJ script for counting intracellular protein aggregates/puncta from a directory containing images obtained with 10x objectives of widefield or confocal fluorescence microscopes. The detection is based on using the ImageJ plugin ComDet and can use images with fluorescently-tagged proteins or after fluorescent antibody labelling. The analysis can be modified to work with different settings by changing the size of analysed particles in the "ch1a_arr" line or/and the intensity threshold (in SD) in the "ch1s_arr" line.

Fields of Study & Subjects

Cell Image Analysis Techniques Advanced Fluorescence Microscopy Techniques Image Processing Techniques and Applications

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
13 January 2026