Substrate specificity of Brucella suis 513 Fructose-1,6-bisphosphatase (Fbp) (A) and Broad spectrum phosphatase (Bbp) (B).

The phosphatase activity was measured in the presence of 0.25 mM of the indicated substrates using a colorimetric assay that quantifies the inorganic phosphate formed during the reaction. The measurements were repeated three times. F1,6bP, fructose 1,6-bisphosphate; S1,7bP, sedoheptulose 1,7-bisphosphate; F1P, fructose 1-phosphate; F6P, fructose 6-phosphate; R5P, ribose 5-phosphate; PEP, phosphoenolpyruvate phosphate; 2PG, 2-phosphoglycerate; 3PG, 3-phosphoglycerate; 2,3PG, 2,3-bisphosphoglycerate; DHAP, dihydroxyacetone-phosphate; D-GAP, D-glyceraldehyde-3-phosphate; D-Gol3P, D-3-phosphoglycerol; L-Gol3P, L-3-phosphoglycerol; D-E4P, D-erythrose 4-phosphate; G1P, glucose 1-phosphate; G6P, glucose 6-phosphate; G1,6bP, glucose 1,6-bisphosphate; 6-PG, 6-phosphogluconate. Statistical significance was evaluated using One-sample t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).