Kinetic properties and specificity of Brucella Broad spectrum phosphatase (Bbp), and Fructose-1,6-bisphosphatases, Fbp and GlpX
Abstract
Values are the mean ± standard error of three technical replicates. Three biological replicates performed did not differ by more than 10%. The activities were measured with Fructose-1,6-bisphosphate (F1,6bP) (spectrophotometric assay) or Sedoheptulose 1,7-bisphosphate (S1,7bP) (Itaya assay) as substrate. Brucella suis GlpX modified to correct inactivating mutations according to the Brucella abortus 2308W sequence (i.e., Bs513GlpX139Gly316Arg).
Fields of Study & Subjects
Bacterial Genetics and Biotechnology
Biochemical and Molecular Research
Brucella: diagnosis
epidemiology
treatment
Publication details
- Journal / source
- Zenodo (CERN European Organization for Nuclear Research)
- Publication date
- 15 January 2026