Brucella Fructose-1,6-bisphosphatase (Fbp) requires Mg2+ for activity and is inhibited by Zn2+ while Broad spectrum phosphatase (Bbp) does neither require Mg2+ for activity nor is inhibited by Zn2+

Fbp (A,C) and Bbp (B,D) phosphatase activity was measured in a spectrophotometric assay in the presence of 0.5 mM Fructose 1,6-bisphosphate (F1,6bP) as substrate and the indicated concentration of metals. The measurements were repeated three times. Statistical significance was evaluated using one-way ANOVA followed by Dunnett’s test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).