Multi-Omics Insights Into Liver Development Alterations Driven by Pre-Weaning Nutrition in Holstein Heifer Calves

Supplementary Information Table S1. Statistics on reads: Reads mapping statistics of RRBS data (A). Reads mapping statistics of RNA-seq data (B). Reads mapping statistics of miRNA-seq data (C). Table S2. Chromosomal distribution of DMSs in the context of CpG and the level of differences in their methylation in liver tissue (A). The list of DMSs in different genetic regions (B). Functional enrichment of DM genes hypermethylated (C) and hypomethylated (D) in the AL group compared to the R group. Table S3. The list of differentially expressed miRNAs identified in the AL group compared to the R group, in the liver tissue (A). Functional enrichment of DE miRNA target genes (B-D). The enriched KEGG pathways involving identified DEmiRNA target genes (E). Table S4. The list of differentially expressed genes (DEGs) identified the AL group compared to the R group (A). Functional enrichment of upregulated (B) and downregulated (C) DEGs. The enriched KEGG pathways involving identified down-regulated DEGs (D). Table S5. Primer sequences for validation of selected DEGs (A) and the results of validation of selected DEGs (B). Table S6. Analysis of correlation between miRNA and target genes expression for all identified DEmiRNAs and DEGs in the liver tissue (A). Functional enrichment of DEmiRNA-related DEGs (B). The enriched KEGG pathways involving identified DEmiRNAs-related DEGs (C).