Ridge regression model for estimating epigenetic passage from ELOVL2 CpG methylation

This repository contains a Python script used to construct and evaluate a ridge regression model for estimating cell passage number from DNA methylation levels at CpG sites within the ELOVL2 locus. The model was trained and evaluated using leave-one-out cross-validation on primary human dermal fibroblast (HDFn) samples spanning passages 2–15. The code accompanies a research manuscript investigating the stability and perturbation of ELOVL2 methylation during in vitro cell proliferation.