theheking/isoform_specific_perturb_seq: v1.0

Using the Direct Capture Perturb-seq technology (Replogle, J.M. et al. Nat Biotechnol 38, 954–961 (2020)), we created a pooled of guides that target sites of promoter usage within gene candidates on a stable MCF-7 KRAB dCas9 cell line. Single-cell sequencing 10x5' with CRISPR library and transcriptome. This is the compilation of the Github repositry used to create the figures. Please access the website here: https://isoform-specific-perturb-seq.readthedocs.io/en/latest/index.html Please access the raw data here: https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-14567?key=244774e5-fc31-4e23-8102-4fbd49b2ed09