Evaluation Of Antioxidant and Enzyme Inhibition Potential of Various Extracts of Mushroom Species

The present study aimed to evaluate the antioxidant and pancreatic lipase inhibitory potentials of ethyl acetate extracts from five mushroom species: Hypsizygus tessellatus, Lentinula edodes, Schizophyllum commune, Tricholoma matsutake, and Coprinellus disseminatus. The mushrooms were collected and extracted using the maceration method, followed by preliminary phytochemical screening to identify the presence of alkaloids, glycosides, phenols, and tannins. Flavonoids were absent in all extracts. Antioxidant activity was assessed using the DPPH radical scavenging assay, which revealed that none of the extracts exhibited significant free radical scavenging potential. In contrast, all mushroom extracts demonstrated notable inhibition of pancreatic lipase in vitro. Among them, Hypsizygus tessellatus exhibited the highest inhibitory activity with an IC50 value of 4.4 µg, followed by Tricholoma matsutake (IC50 = 6.94 µg), Schizophyllum commune (IC50 = 11.27 µg), Lentinula edodes (IC50 = 17.58 µg), and Coprinellus disseminatus (IC50 = 17.7 µg). These findings suggest that the studied mushrooms, particularly Hypsizygus tessellatus, possess potent pancreatic lipase inhibitory activity, highlighting their potential as natural agents for managing obesity and related metabolic disorders