19F-NMR spectra of artificial 17-OPH biosensor (cpOHPFA_1952-20-BLA-253) in apo and holo states

This dataset contains 19F-NMR spectra supporting Figure 3e, Figure 3f, and Supplementary Figure 10 of the manuscript "Artificial allosteric protein switches with machine learning-designed receptors". The data consists of spectra for the cpOHPFA_1952-20-BLA-253 chimera, an artificial allosteric biosensor composed of a machine learning-designed 17alpha hydroxy-progesterone (17-OPH) binding domain inserted into TEM-1 beta-lactamase. To probe conformational dynamics, p-(O)CF3-tyrosine was installed at specific positions (including 105, 229, 230, 256, 389, and 437) via in vivo genetic code expansion.