INVESTIGATION OF CONSTITUENTS AND BIOLOGICAL ACTIVITY AND GC-MS ANALYSIS OF OIL FROM SAUDI ARABIA SPECIES: LACTUCA SATIVA

The present study was designed to investigate the chemical constituents of Saudi Lactuca sativa seed oil and to evaluate its potential antimicrobial activity. 20 components were detected by GC-MS analysis. major constituents are: 6- Octadecenoic acid, methyl ester, (Z)- (64.01%), 9,12-Octadecadienoic acid (Z,Z)-, methyl ester(13.21%), Carotol (9.92%), Hexadecanoic acid, methyl ester (6.50%), Methyl stearate (1.40%) and Daucol (1.26%). The antimicrobial activity of the oil was evaluated via cup plate agar diffusion assay against five standard human pathogens (gram negative :staphylococcus aureus, bacillus subtilis and the fungi Candida albicans, gram positive: Escherichia coli and Pseudomonas aeruginosa. Lactuca sativa oil showed good activity against Escherichia coli and Pseudomonas aeruginosa. However, it was partially inactive against other test organisms. Percentage radical scavenging activity by samples was good active.