Membrane protein solubilization and structure determination using de novo-designed amphipathic proteins

Zenodo (CERN European Organization for Nuclear Research) 2026 Open access

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Authors: Ljubica Mihaljević, David E. Kim, Pooja Bandawane, Helen E. Eisenach, Andrew J. Borst, Alexis Courbet, Connor Weidle, Kenneth D. Carr, Everton Burlamarque Bettin, Qiushi Liu, Aldo T. Trejos, Sagardip Majumder
+21 more Surabhi Kokane, Alexander Stevens, Edin Muratspahić, Thomas Schlichthaerle, Marc Expòsit, Xinting Li, Mila Lamb, A G Murray, Rashmi Ravichandran, Elizabeth C. Williams, Hu Shuyuan, Lynda M. Stuart, Linda Grillová, Nicholas R. Thomson, Michael Landreh, Pengxiang Chang, Lorenzo Giacani, Melissa J. Caimano, Kelly L. Hawley, Neil P. King, David A. Baker
DOI: 10.5281/zenodo.18441682
Cited by: 0

Abstract

WRAPs (Water-soluble RFdiffused Amphipathic Proteins) are genetically encoded de novo proteins that surround the lipid-interacting hydrophobic surfaces of transmembrane proteins, rendering them thermostable and water-soluble without the need for detergents. This repo includes scripts and inputs to generate WRAPs as described in https://www.biorxiv.org/content/10.1101/2025.02.04.636539v1.

Fields of Study & Subjects

Protein Structure and Dynamics Lipid Membrane Structure and Behavior Enzyme Structure and Function

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
31 January 2026