Supplementary material and data for FloBuzz: A modular feeder system for automated aversive conditioning in bees

Supplementary material and data for publication: Gultekinoglu E., Erdem, B., Botasun, A., Arslan, O. C., Sevin, S., Gozen, A. G., Alemdar, H., Sahin, E., & Turgut, A. E. (2026). FloBuzz: a modular feeder system for automated aversive conditioning in bees. bioRxiv. https://doi.org/10.64898/2026.02.08.704633 inTrigger_Codes.zip includes the scripts of the inTrigger module: - In_Trigger_GUI.py is the user interface of the In-Trigger module. - In_Trigger_Core.py is the core machine code of the In-Trigger module. - In_Trigger_Arduino.ino is code for the microprocessor of the shock switch. - In_Trigger_ReadMe is the information and usage manual of the In-Trigger module. - ShockSwitchDiagram.pdf is the circuit diagram to build the shock switch. Feeder_CAD_Files.zip includes the printable 3D components of the feeder: - Cap.stl is the STL file for the cap of the feeder module.- CollectingBox.stl is the STL file for the collecting box of the feeder module.- ElectricShockGrid.stl is the STL file for the electric shock grid of the feeder module.- MainBase.stl is the STL file for the main base of the feeder module. - FloBuzzTestSetup.f3z is the assembled Fusion 360 file of the complete experimental setup, provided as a representative system-level configuration. SyringePump_Codes.zip includes the necessary files to build the pump control unit.- SyringePump.ino contains the firmware running on the microcontroller of the pump control unit.- SyringePumpLogger.py is used to record operational logs generated by the syringe pump during runtime.- ReadMe.txt describes the basic features of the syringe pump and provides usage instructions.- PumpControlUnitPCB.jpg shows the circuit diagram of the custom-designed PCB for the pump control unit. FloBuzz_Stat.zip includes statistics scripts and experimental data.- FloBuzz_statistics.Rdm is a statistical analysis script to evaluate experimental data in the R language.- FloBuzz_data.xlsx includes experimental data. The data was prepared by manually scoring the 450-second video recording of the electric shock-avoidance experiment at 5-second intervals. The number of bees on the flower and whether the electric shock was active were noted.