Raman microscopy of live Schizosaccharomyces pombe yeast

We acquired coherent Raman microscopy data on fission yeast Schizosaccharomyces pombe (wild-type) and two mutants, dea2 and pdf1. Our aim was to collect spatially resolved biochemical data on living yeast cells. The image preview (rgb_overlay_large.png) is a composite of two single-wavenumber SRS acquisitions, taken at 2,850 (green) and 2,937 cm−1 (blue). This record contains the raw LIF files and metadata in JSON and CSV format. This release accompanies the pub "Coherent Raman imaging of live, unfixed Schizosaccharomyces pombe and mutants dea2 and pdf1", and the GitHub repository for visualizing the dataset and exporting images and metadata is here.