A Comprehensive Review: Simultaneous Estimation of Lamivudine and Silymarin by UV Spectroscopy

Background: UV Spectroscopy are analytical method a rapid and accurate technique to has been developed for simultaneous estimation of lamivudine and silymarin. The absorbance maximum for lamivudine and silymarin were observed at 270.4 nm and 324. 4nm. Lamivudine act as a nucleoside reverse transcriptase inhibitor (NRRI). They act as against HIV. Silymarin drug is used for treatment of chronic liver disease and hepatitis. Silymarin is hepatoprotective agent. Methods: Spectroscopy is the branch of science dealing with the study of interaction of electromagnetic radiation with matter. The technique of U.V. Spectroscopy is one of the most frequently employed in pharmaceutical analysis. It involves the measurement of the amount of ultraviolet (190-380nm) or visible (380-800nm) radiation absorbed by a s substance in solution. U.V. Spectroscopy instrumental method principle based on Beer’s-Lambert’s law. This study revealed that simultaneous iso-absorptive point was 284.0 nm both drugs are almost equal absorbance at this wavelength and also plotted Calibration curve of both drug concentration versus absorbance. Various methods of producer are used: i. Sample Preparation ii. Selection of Wavelength iii. Construction of calibration curves iv. Analysis of sample. Result: This comprehensive review study crucial for simultaneous estimation of lamivudine and silymarin by uv spectroscopy. The absorption maxima of lamivudine were found to be at 270.4nm, and this method obeyed Beer’s law in the concentration range is 5-30𝜇𝑔/𝑚𝑙. The limit of detection and quantification values for lamivudine were 0.043 and 0.130𝜇𝑔/𝑚𝑙. For silymarin absorbance maxima were found to be at 324.4nm, and the method obeyed beer’s law in the concentration range is 5-30𝜇𝑔/𝑚𝑙. The limit of detection and quantification value for silymarin were 0.129 and 0.392𝜇𝑔/𝑚𝑙. Conclusion: Spectroscopy analytical method is a most valuable, simple, specific, accurate, precision and rapid technique for pharmaceutical analysis of these two drugs. The method obeys Beer’s law and has a good limit of detection and quantification value. Simultaneous estimation of both drug lamivudine and silymarin by uv spectroscopy involves using specific wavelength to determine the concentration of both drugs.