Arp2/3-dependent actin assembly shapes endosomes and promotes intracellular trafficking in fission yeast

Arp2/3-dependent actin assembly shapes endosomes and promotes intracellular trafficking infission yeast Alejandro Melero&, 1, %, Miguel Basante-Bedoya&, 2, Olivia Muriel-Lopez1,‡ and Sophie G Martin1,2, * 1 Department of Fundamental Microbiology, University of Lausanne, Switzerland2 Department of Molecular and Cellular Biology, University of Geneva, Switzerland% Current address: i+Med S. Coop, Parque Tecnologico de Alava, Vitoria-Gasteiz, Spain‡ Current address: National Center for Biotechnology, Madrid, Spain.& These authors contributed equally to this work.*Correspondence: Sophie.Martin@unige.chhttps://www.biorxiv.org/content/10.1101/2024.03.28.587130v1.abstract Tomography and correlation files corresponding to figure 2 and S3. Each folder contains the .tif file of tomographic reconstruction, and a FigureX_Correlation.tif file with the correlation of fluorescence signal and electron microscopy. Pixel size 1.205 nm Project_Root/├── Figure2/│ ├── Fig2_Tomogram.tif # Unprocessed tomographic reconstruction│ └── Fig2_Correlation.tif # Multichannel fluorescence overlaid on EM└── FigureS3/ ... Tomograms: Unprocessed .tif stacks converted from IMOD .rec files via mrc2tif command. Correlation Files:Green: GFP-Syb1 Magenta: mCherry-D4H Blue: Dapi Alignment: All channels are registered to an EM slice using TetraSpeck fiducials (visible in all channels) and gold fiducials (15 nm)