Processed snRNA-seq Seurat object for kidneys of the diabetic kidney disease GIPRdn-tg mouse model treated ± FC-insulin

This dataset contains a processed snRNA sequencing dataset stored as a Seurat object (.rds), with normalized gene expression data, cell-level metadata, dimensionality reductions (PCA and UMAP), and cell-type annotations. Organism: Mus Musculus, CD1 background Tissue: KidneyMice: The data were generated using the GIPR dominant negative transgenic mouse model that recapitulates key features of human diabetic kidney disease (DKD). The study assessed transcriptional responses during diabetic conditions and following glycemic normalization using a novel ultra long acting Fc conjugated insulin analog. At 7 weeks of age, wildtype mice received vehicle in parallel to GIPRdn-tg mice (reference see below), which were split into insulin and vehicle groups. Insulin-treated mice received an initial dose of 40 nmol/kg ultra-long-acting insulin (compound 12), adjusted weekly (35–20 nmol/kg) until a maintenance dose (20–25 nmol/kg) was reached. From treatment weeks 5–16, insulin was administered weekly, with glucose monitored daily initially, then twice weekly. (Reference: Herbach, N., Schairer, I., Blutke, A., Kautz, S., Siebert, A., Göke, B., Wolf, E., and Wanke, R. (2009). Diabetic kidney lesions of GIPRdn transgenic mice: podocyte hypertrophy and thickening of the GBM precede glomerular hypertrophy and glomerulosclerosis. Am J Physiol Renal Physiol 296, F819-829. 10.1152/ajprenal.90665.2008. Sample Collection and Nuclei Isolation: At 23 weeks of age, kidneys were perfusion fixed, and a 1 mm transverse mid section containing cortex, outer/inner medulla, and papilla was collected. After trimming ~1 mm cortex from both sides, tissue sections were snap frozen for nuclei extraction. For each group (4 WT, 4 GIPRdn tg Vehicle, 4 GIPRdn tg Insulin), frozen kidney slices were homogenized in ice cold nuclei isolation buffer (0.25 M sucrose, 25 mM KCl, 5 mM MgCl₂, 20 mM Tris pH 8.0, 0.4% IGEPAL-630, 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, phosphatase/protease inhibitors, and RNase inhibitors). Tissue was mechanically dissociated using loose and tight Dounce pestles, filtered (100 μm → 20 μm), and centrifuged at 1000 × g, 10 min, 4°C. The nuclei pellet was resuspended in DAPI containing staining buffer and inspected for integrity using fluorescence microscopy. Fluorescence Activated Nuclei Sorting (FANS): Nuclei were sorted on a BD FACSAria III using a 70 μm nozzle. Single nuclei were gated using:• side scatter height vs. area• DAPI intensity for doublet exclusionSorted nuclei were collected in nuclei buffer (