Official Publication: Genomic Innovation in Precision Oncology – Integrated CRISPR-TTP Architecture for Ewing Sarcoma (Version 4.0)

Official technical specification and supplementary data for the peer-reviewed article: "Genomic Innovation in Precision Oncology: Integrated CRISPR-TTP Bioengineering Architecture for Ewing Sarcoma (Version 4.0)" published in Frontiers in Genetics (2026). Abstract: This record contains the complete architectural blueprint and standardized operating procedures (SOP v2.0) for the Althea Framework. The architecture integrates high-fidelity CRISPR-Cas9 genome engineering, MR-guided Focused Ultrasound (FUS) triggered delivery, and AI-driven therapeutic orchestration. Key Components Included: Full Architectural Specification (v4.0): Detailed logic of the integrated bioengineering system. Clinical Workflow: A 21-day GMP-ready pathway for autologous dendritic cell manufacturing and nanoparticle administration. In Silico Validation Data: Predictive modeling results for tumor growth inhibition and immune response dynamics. AI Co-pilot Framework: Methodology for real-time personalization and target optimization. License: Released under CC BY 4.0. This work is intended to serve as an open-source survival infrastructure for treatment-resistant fusion-driven malignancies.