Fiji/Python script for quantification of ice diffraction spots in cryo-electron microscopic tilt series

Zenodo (CERN European Organization for Nuclear Research) 2026 Open access

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Authors: Thorsten Wagner, Elisa Lisicki, Tatjana Taubitz, Stefan Raunser, Sebastian Tacke
DOI: 10.5281/zenodo.18608945
Cited by: 0

Abstract

Repository contents README.md: Installation and usage instructions. ice_counter.ijm: Analysis script stats.py: Statistics script 2500nA_n1_l35t01.mrc & 2500nA_n1_l50t01.mrc: Example input dataset, consisting of a devitrified tilt-series (2500nA_n1_l35t01.mrc) and a vitrified tilt-series (2500nA_n1_l50t01.mrc). 2500nA_n1_l35t01_counts.csv & 2500nA_n1_l50t01_counts.csv: Expected output generated by ice_counter.ijm from the test dataset. stats.csv: Expected output generated by stats.py from 2500nA_n1_l35t01_counts.csv & 2500nA_n1_l50t01_counts.csv.

Fields of Study & Subjects

Advanced Electron Microscopy Techniques and Applications Enzyme Structure and Function Advanced Materials Characterization Techniques

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
11 February 2026
Language
EN