Vishniacozyma pseudofoliicola A. N. Poliakova, A. V. Kachalkin 2026, sp. nov.

Vishniacozyma pseudofoliicola A. N. Poliakova, A. V. Kachalkin sp. nov. Fig. 3 A Etymology. The name Vishniacozyma pseudofoliicola (pseu. do. fo. li. i’co. la. N. L. fem. adj.) refers to a similarity with V. foliicola. Description. When growing on 5 % malt extract agar (MEA) after 7 days at 25 ° C, the streak culture is butyrous, glistening, smooth and flat, with an entire margin, ivory to faint beige in color with a slightly lighter color at the margin. The color darkens with age, the lighter margin is retained. On potato dextrose agar (PDA) after 7 days at 25 ° C, the streak culture is same as on MEA, but slightly raised and with uniform color. Cells are globose and ovoid (2.5–6.6 × 3.6–7.5 µm) and occur singly or in pairs (Fig. 3 A). Budding is multilateral. Ballistoconidia are not produced. Mycelium, pseudomycelium and sexual structures (mating test) are not observed on MEA, PDA, corn meal agar (CMA) and V 8 agar at 10 ° C and 25 ° C over a period of two months. Glucose is not fermented. Positive growth on glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melizitose, D-xylose, L-arabinose, D-arabinose (weak and slow), D-ribose (weak and variable), L-rhamnose, erythritol, ribitol (weak and variable), myo-inositol, methyl-α-d-glucoside, D-glucuronic acid (positive or slow), DL-lactic acid (weak), succinic acid (positive or slow), citric acid (slow or weak), 2 - Keto-D-gluconate, 5 - Keto-D-gluconate, arbutin. No growth on sorbose, inulin, soluble starch, D-glucosamine, methanol, ethanol, glycerol, galactitol, D-mannitol, glucitol, salicin, and hexadecane. Ammonium sulphate, lysine and D-glucosamine are assimilated as sole nitrogen sources, but potassium nitrate, ethylamine hydrochloride and creatine are not assimilated. Growth in the vitamin-free medium is positive. Extracellular starch-like compounds are produced. Weak growth may occur (variable test) in 10 % (w / v) sodium chloride with 5 % (w / v) glucose medium, but no at 16 % (w / v) sodium chloride. Weak growth may occur in the presence of 0.01 % cycloheximide, but growth is absent with 0.1 % cycloheximide. Growth on 50 % (w / v) glucose-yeast extract agar is positive. Urea is hydrolyzed, and the color reaction with diazonium blue B is positive. The maximal growth temperature is 28 ° C. Typus. Russia • Voronezh Region: Voronezh State Natural Biosphere Reserve by V. M. Peskov, from a Haplic Gleysols Humic soil, November 1966, А. V. Kartintsev, KBP 1600 (holotype KBP Y-7396 T preserved as a metabolically inactive state, cultures ex-type DBVPG 8080 and VKM Y-3715). Additional strain examined. Russia • Moscow Region: settlement of Kosmodem’yanski, from the frass of Scolytus scolytus larvae under the bark of elm tree, August 2023, A. V. Kachalkin, KBP Y-7320 (= VKM Y-4103). GenBank accession numbers. Holotype KBP Y-7396 T (ITS and D 1 / D 2 LSU: OP 602955; RPB 1: PV 029737; RPB 2: PV 029733; TEF 1-α: PV 029735); additional strain KBP Y-7320 (ITS and D 1 / D 2 LSU: OR 623241; RPB 1: PV 029738; RPB 2: PV 029734; TEF 1-α: PV 029736).