Vishniacozyma kombuchae A. N. Poliakova, A. V. Kachalkin 2026, sp. nov.

Vishniacozyma kombuchae A. N. Poliakova, A. V. Kachalkin sp. nov. Fig. 3 B Etymology. The name Vishniacozyma kombuchae (kom. bu'chae. N. L. gen. fem. n.) refers to the isolation of the type strain from the kombucha tea. Description. When growing on 5 % malt extract agar (MEA) and on potato dextrose agar (PDA) after 7 days at 25 ° C, the streak culture is mucoid, glistening, smooth and flat, with an entire margin, faint beige in color. The color darkens with age. Cells are globose, ovoid and ellipsoidal (1.6–6.0 × 3.0–7.5 µm) and occur singly or in pairs (Fig. 3 B). Budding is polar. Ballistoconidia are not produced. Mycelium, pseudomycelium and sexual structures are not observed on MEA, PDA, corn meal agar (CMA) and V 8 agar at 10 ° C and 25 ° C over a period of two months. Glucose is not fermented. Positive growth on glucose, galactose, sorbose (slow), sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melizitose, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine (slow), erythritol, ribitol (weak), galactitol, D-mannitol, glucitol, myo-inositol, methyl-α-d-glucoside, salicin (weak), DL-lactic acid (weak), D-glucuronic acid, 5 - Keto-D-gluconate and arbutin (weak). No growth on inulin, soluble starch, methanol, ethanol, glycerol, succinic acid, citric acid, and hexadecane. Ammonium sulphate, lysine and D-glucosamine are assimilated as sole nitrogen sources, but potassium nitrate, ethylamine hydrochloride and creatine are not assimilated. Growth in the vitamin-free medium is negative. Extracellular starch-like compounds are produced. Weak growth in 10 % (w / v) sodium chloride with 5 % (w / v) glucose medium, but no at 16 % (w / v) sodium chloride. Weak growth may occur in the presence of 0.01 % cycloheximide, but growth is absent with 0.1 % cycloheximide. Growth on 50 % (w / v) glucose-yeast extract agar is weak and variable. Urea is hydrolysed, and the color reaction with diazonium blue B is positive. The maximal growth temperature is 30 ° C. Typus. Russia • Moscow: from home-made kombucha tea, September 2024, A. N. Poliakova, KBP Y-7350 (holotype KBP Y-7350 T preserved as a metabolically inactive state, cultures ex-type DBVPG 8078 and VKM Y-4117). GenBank accession numbers. Holotype KBP Y-7350 T (ITS and D 1 / D 2 LSU: PP 294695; RPB 1: PV 029730; RPB 2: PV 029731; TEF 1-α: PV 029732).