Vishniacozyma fructicola A. N. Poliakova, A. M. Glushakova, A. V. Kachalkin 2026, sp. nov.

Vishniacozyma fructicola A. N. Poliakova, A. M. Glushakova, A. V. Kachalkin sp. nov. Fig. 3 C Etymology. The name Vishniacozyma fructicola (fruc. ti'co. la. N. L. masc. n.) refers to isolation of the type strain from the fruit of cornel. Description. When growing on 5 % malt extract agar (MEA) and on potato dextrose agar (PDA) after 7 days at 25 ° C, the streak culture is butyrous, glistening, smooth and flat, with an entire margin, faint beige to beige in color with a slightly lighter color at the margin. Cells are ovoid, ellipsoidal to elongate (1.8–4.2 × 3.8–7.0 µm) and occur singly or in pairs (Fig. 3 C). Budding is mainly polar but can also be bipolar or multilateral. Ballistoconidia are not produced. Mycelium, pseudomycelium and sexual structures are not observed on MEA, PDA, corn meal agar (CMA) and V 8 agar at 10 ° C and 25 ° C over a period of two months. Glucose is not fermented. Positive growth on glucose, sorbose (weak), sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melizitose, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, glycerol, erythritol, ribitol, D-mannitol, glucitol, myo-inositol, methyl-α-d-glucoside, salicin, D-glucuronic acid (weak), DL-lactic acid (weak), succinic acid, 2 - Keto-D-gluconate, 5 - Keto-D-gluconate, and arbutin. No growth on inulin, soluble starch, methanol, ethanol, galactitol, citric acid. Ammonium sulphate, potassium nitrate, lysine, creatine, creatinine, and D-glucosamine are assimilated as sole nitrogen sources, but ethylamine hydrochloride and cadaverine are not assimilated. Growth in the vitamin-free medium is positive. Extracellular starch-like compounds are produced. Growth in 10 % (w / v) sodium chloride with 5 % (w / v) glucose medium, but no at 16 % (w / v) sodium chloride. Weak growth may occur in the presence of 0.01 % cycloheximide, but growth is absent with 0.1 % cycloheximide. Growth on 50 % (w / v) glucose-yeast extract agar is positive. Urea is hydrolysed, and the color reaction with diazonium blue B is positive. The maximal growth temperature is 29 ° C. Typus. Russia • Moscow Region: city of Electrougli, from cornel fruits, July 2019, A. M. Glushakova, KBP YE-0008 (holotype KBP Y-6599 T preserved as a metabolically inactive state, cultures ex-type DSM 110869 and VKM Y-3606). GenBank accession numbers. Holotype KBP Y-6599 T (ITS and D 1 / D 2 LSU: MT 013023; RPB 1: PV 061054; RPB 2: PV 061055; TEF 1-α: PV 061056). Note. Vishniacozyma fructicola has been placed in clade (Fig. 1) includes isolate PB 780 (GenBank KX 078413) from fermenting wine in Italy, which is the same species.